• LC-MS/MS analysis of PFAS in human serum
    Fig 1: Separation of 28 PFAS using a YMC-Triart C18 column as an analytical column.


LC-MS/MS analysis of PFAS in human serum

Nov 23 2021

Analysis of PFAS using a YMC-Triart C18 column in combination with an online SPE column-switching method

Poly- and perfluoroalkyl substances (PFAS) are widely used because of their special properties which include heat resistance and water and fat repellency. Commonly known products in which PFAS can be found include non-sticky cookware, water-repelling textiles or glossy magazines. More than 4,000 substances are available on the market, which all are industrially originated.

Due to their molecular structure which contains carbon-fluorine bonds, PFAS benefit from a high stability, both thermal as well as chemical. However, this stability also leads to a persistency in the environment as they are not fully degradable which makes them persistent organic pollutants (POPs). These POPs not only accumulate in the environment but also in the human body since they are absorbed from food, water or air.

As a result, they are found predominantly in human blood or organs, which is why they need to be strictly monitored and regulated. As they are recently been assumed to be potentially carcinogenic, the daily uptake limit was lowered especially for two substances. Due to the adverse health effects of PFAS, a highly sensitive method is of particular relevance.

Automated SPE coupled to an online SPE column switching LC-MS/MS method

This application note shows the analysis of 28 PFAS in human serum, based on the study of Nakayama et al. An automated solid phase extraction (SPE) system was coupled to an online SPE column-switching LC-MS/MS method. Anion-exchanging scrubber columns were placed downstream of the LC pumps A, C and D in order to eliminate possible contamination from the system. A YMC-Triart C18 column used as the analytical column shows excellent results for this sensitive analysis.

Digital Edition

Chromatography Today - Buyers' Guide 2022

October 2023

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


China Lab 2024

Mar 05 2024 Guangzhou, China

analytica 2024

Apr 09 2024 Munich, Germany

Korea Lab 2024

Apr 23 2024 Kintex, South Korea

Korea Chem 2024

Apr 23 2024 Seoul, South Korea

AOCS Annual Meeting & Expo

Apr 28 2024 Montreal, Quebec, Canada

View all events