Rapid Determination of Myo-Inositol in Infant Formula and Adult Nutritionals

Thermo Fisher Scientific has developed a high-performance anion-exchange chromatography with pulsed amperometric detection method, based on AOAC Official Method 2011.18, to determine free and bound myo-inositol in infant formula and adult nutritionals. Application Note (AN) 1083: Determination of Myo-Inositol (Free and Bound as Phosphatidylinositol) in Infant Formula and Adult Nutritionals demonstrates a column-switching technique used to remove strongly retained carbohydrates in the sample, thereby reducing run time. Pulsed amperometric detection with a gold on polytetrafluoroethylene disposable electrode offers high sensitivity while eliminating the need for sample derivatization and electrode polishing.

For the determination of bound myo-inositol, AN 1083 compares the results obtained using a silica solid-phase extraction (SPE)column as described in AOAC Official Method 2011.18 to using a polymeric reversed-phase sample pretreatment cartridge, the Thermo Scientific^™ Dionex^™ OnGuard^™ II RP cartridge. The results demonstrate that the pretreatment cartridge produces higher content and provides improved recoveries of bound myo-inositol in the samples. The pretreatment cartridge also reduces sample preparation time and eliminates the hazardous chemicals associated with use of the silica SPE column.

This application brief and many others can be found at www.thermoscientific.com/dionex under the Documents tab.