Rapid Determination of Myo-Inositol in Infant Formula and Adult Nutritionals
Jul 25 2014
Thermo Fisher Scientific has developed a high-performance anion-exchange chromatography with pulsed amperometric detection method, based on AOAC Official Method 2011.18, to determine free and bound myo-inositol in infant formula and adult nutritionals. Application Note (AN) 1083: Determination of Myo-Inositol (Free and Bound as Phosphatidylinositol) in Infant Formula and Adult Nutritionals demonstrates a column-switching technique used to remove strongly retained carbohydrates in the sample, thereby reducing run time. Pulsed amperometric detection with a gold on polytetrafluoroethylene disposable electrode offers high sensitivity while eliminating the need for sample derivatization and electrode polishing.
For the determination of bound myo-inositol, AN 1083 compares the results obtained using a silica solid-phase extraction (SPE)column as described in AOAC Official Method 2011.18 to using a polymeric reversed-phase sample pretreatment cartridge, the Thermo Scientific™ Dionex™ OnGuard™ II RP cartridge. The results demonstrate that the pretreatment cartridge produces higher content and provides improved recoveries of bound myo-inositol in the samples. The pretreatment cartridge also reduces sample preparation time and eliminates the hazardous chemicals associated with use of the silica SPE column.
This application brief and many others can be found at www.thermoscientific.com/dionex under the Documents tab.
Chromatography Today - Buyers' Guide 2022
In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...
View all digital editions
Apr 18 2023 Kintex, South Korea
Apr 19 2023 Ho Chi Minh City, Vietnam
Apr 30 2023 Dublin, Ireland & Online
Apr 30 2023 Denver, CO, USA
May 09 2023 Hannover, Germany