Autosamplers

Protein Applications for Advanced Multi-Detector Size-Exclusion Chromatography

Author: by Mark Pothecary, Stephen Ball, Paul Clarke on behalf of Malvern Instruments Ltd

Free to read

This article has been unlocked and is ready to read.

Download

Size-exclusion chromatography (SEC) or gel permeation chromatography (GPC) is frequently used in bioscience laboratories to characterise purified and recombinant proteins. This technique has also been used to measure molecular weight or to resolve mixtures of monomers, oligomers and higher order aggregates. Using this method, molecular weight is calculated by creating a calibration curve of standard globular proteins and comparing the elution time of an unknown to the standards. This is limited in that it is based on a stable relationship between molecular weight, size, shape or hydrodynamic volume. This relationship varies significantly between proteins introducing unquantifiable inaccuracies in all measurements made in this way.

Free to read

This article has been unlocked and is ready to read.

Download


Events

ASMS Conference

Jun 01 2025 Baltimore, MD, USA

HPLC 2025

Jun 15 2025 Bruges, Belgium

LabAsia 2025

Jul 14 2025 Kuala Lumpur, Malaylsia

SinS Solutions in Science

Jul 15 2025 Brighton, UK

ACS National Meeting - Fall 2025

Aug 17 2025 Washington DC, USA & Virtual

View all events