• Fast and Easy Achiral & Chiral Analysis of Cannabinoids

HPLC, UHPLC

Fast and Easy Achiral & Chiral Analysis of Cannabinoids

May 20 2019

It has been repeatedly suggested that the effect of Δ9-Tetrahydrocannabinol (THC) or other single cannabinoids are not equal to that of whole cannabis preparations: some of the bio-activity observed for these preparations could be due to acidic cannabinoids. That is why a method which separates both neutral compounds such as THC as well as acidic cannabinoids such as THCA in plant material must be available.

In Europe there is a growing interest in medicinal cannabis, which is used to treat a series of pathologies. Nonetheless, cannabis is a strictly controlled drug under specific regulations or is illegal even for therapeutic purposes in many European countries. Certified medicinal cannabis is currently used for the treatment of a number of pathologies, including multiple sclerosis, epilepsy, neuropathic pain, arthritis, depression, anxiety disorders, sleep disorders, psychosis, glaucoma and Tourette’s syndrome. It is also used for the relief of symptoms such as nausea and vomiting as a result of chemotherapy, and appetite stimulation in patients suffering from HIV and AIDS.

The cannabinoids form a group of related compounds of which about 70 are known. Among the major cannabinoids in Cannabis sativa L. THC is generally accepted to be the compound that possesses the psychoactive properties.

The analysis of the original composition of plant material is necessary for such purposes as phenotyp determination and quality control of medical cannabis used for therapeutic treatment. HPLC permits the determination of the original composition of plant cannabinoids by direct analysis.

This application note describes two fast methods for separating different cannabinoids using YMC-Triart C18 UHPLC column and YMC’s Core-Shell Meteoric Core C18 column. A third rapid method shows a method for the chiral separation of THC isomers using the polysaccharide CHIRAL ART Amylose-C column.


Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

ACS National Meeting & Expo, Fall 2022

Aug 21 2022 Chicago, IL, USA & Online

ACHEMA 2022 - NEW DATES

Aug 22 2022 Frankfurt, Germany

IMSC 2022

Aug 27 2022 Maastricht, Netherlands

EuCheMS Chemistry Congress

Aug 28 2022 Lisbon, Portugal

JASIS 2022

Sep 07 2022 Chiba, Japan

View all events