• Expert Tip: (U)HPLC Method Parameter Quick Reference

HPLC, UHPLC

Expert Tip: (U)HPLC Method Parameter Quick Reference

Jun 21 2022

When choosing a (U)HPLC column for an application the most important factor for high resolution is selectivity. This determining factor is mostly dependent on the analytes of interest but it can be altered, for example, by the choice of the stationary phase modification as well as organic solvents and additives of the mobile phase. However other important parameters such as:

  • column inner diameter or radius
  • column length
  • stationary phase particle diameter
  • mobile phase flow rate
  • injection volume
  • porosity of sample or mobile phase filters

are all interconnected and need to be considered to achieve optimal resolution. Especially when transferring method between different types of systems or detection methods, adjustments of these parameters are unavoidable.

This new Expert Tip by YMC provides a quick reference of common combinations for column dimensions and suitable method parameters to help chromatographers in method transfer, scaling, optimisation and troubleshooting. You will find practical aspects and overview tables how to:

  • adjust flow rate to different stationary phase particle sizes and column ID
  • adjust sample injection volume to total column volume and solvent strength
  • preserve column life time.

Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions

Events

ICMGP 2022

Jul 24 2022 Virtual event

ACS National Meeting & Expo, Fall 2022

Aug 21 2022 Chicago, IL, USA & Online

ACHEMA 2022 - NEW DATES

Aug 22 2022 Frankfurt, Germany

IMSC 2022

Aug 27 2022 Maastricht, Netherlands

EuCheMS Chemistry Congress

Aug 28 2022 Lisbon, Portugal

View all events