• Trouble Shooting for Liquid Chromatography.


Trouble Shooting for Liquid Chromatography.

Jan 24 2023

Liquid chromatography (LC) is a powerful analytical tool that has become an essential part of many scientific laboratories. However, like any technique, it is not without its challenges, and troubleshooting can be a significant part of the work of an LC analyst.

In recent years, there have been some significant advances in our understanding of how pressure affects retention in LC. These insights are leading to a change in the way we interpret LC results and approach troubleshooting with modern instruments.

One key discovery is that pressure can significantly affect retention times in LC separations. This has important implications for the accuracy of our results, as variations in pressure can lead to changes in the retention of different compounds. This can make it more difficult to identify and quantify compounds in a sample, especially when working with complex mixtures.

To address this issue, modern LC instruments are equipped with more precise pressure control systems. These systems allow us to better maintain consistent pressure during a separation, reducing the impact of pressure on retention times. This can help to improve the accuracy and reproducibility of our results.

In addition to pressure, there are many other factors that can affect the performance of an LC system. These include temperature, pH, and the choice of mobile phase. Careful control of these variables is essential to ensure that our results are reliable and accurate.

To troubleshoot problems with an LC system, it is important to have a systematic approach. This may involve checking for issues with the instrument itself, such as leaks or blockages, as well as reviewing the experimental conditions and method. By systematically working through potential issues, it is often possible to identify and resolve problems with an LC system.

In conclusion, the advances in our understanding of the effects of pressure on retention in LC are changing the way we approach troubleshooting with modern instruments. By maintaining precise pressure control and carefully considering all variables that may affect performance, we can improve the reliability and accuracy of our LC results.

More information online

Digital Edition

Chromatography Today - Buyers' Guide 2022

December 2021

In This Edition Modern & Practocal Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr...

View all digital editions


Plastics Recycling World Expo Europe

Jun 14 2023 Essen, Germany

HPLC 2023

Jun 18 2023 Dusseldorf, Germany


Jul 04 2023 Cardiff, UK

Water Expo Nigeria 2023

Jul 11 2023 Lagos, Nigeria

ACS National Meeting & Expo, Fall 2023

Aug 13 2023 San Francisco, CA, USA

View all events