New Era in Supercritical Fluid Chromatography
The performance and benefits of supercritical fluid chromatography (SFC) have been recognized already for decades. Due to the introduction of the latest generations of SFC systems, instrument robustness as well as system suitability testing are expected to soon reach the same levels of gas chromatography (GC) and high performance liquid chromatography (HPLC). Consequently, the technology has regained increased interest for a variety of applications in a wide range of industries.
From a fundamental standpoint, SFC is characterized by high resolution and high optimal mobile phase velocities. Next to these benefits, SFC is highly versatile and tunable by a variety of parameters including stationary phase, flow rate, pressure, temperature, percentages and nature of modifier and the use of additives. Furthermore, the use of super (or sub) critical CO2 as the bulk of the mobile phase is highly environmental friendly and cost effective, especially when the separations are in a later stage scaled up from analytical to (semi)preparative applications. The niche application for SFC is chiral separations, which have proven to work better in SFC than in LC. SFC can be regarded as a valuable tool within any analytical laboratory as it offers high complementarity towards RPLC and offers an alternative to HILIC for the analyses of non-retained compounds on an octadecyl column.
During this e-learning, the principle of SFC is explained as well as the influence of the different parameters on selectivity. The advantages and bottle necks of the technology are presented and are illustrated with practical examples.
This e-learning will allow you to understand:
- Fundamentals of SFC technology
- Parameters which have an impact on separation in SFC
- Applications: niche and beyond
This session is now ONDEMAND>> and the further learning value and structure conforms to CPD guidelines.
This content has been certified by the CPD Certification Service as conforming to continuing professional development principles.
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This is a free OnDemand session
Isabelle François (WATERS)
Isabelle François conducted her PhD research in the lab of Prof. Pat Sandra at Ghent University in Belgium. During her research, she developed two-dimensional comprehensive fluid-based chromatography approaches (LCxLC and SFCxLC), focusing on the improvement of instrumental hardware. In 2009, she started working at ExxonMobil Chemical managing the chromatography lab in the Advanced Characterization Department. Since 2011, she works for Waters, currently as Business Development Manager for UPC²/SFC and Strategic Technologies covering Europe and India. Her areas of expertise are UHPLC, SFC, comprehensive and heartcutting 2D chromatography (LCxLC, SFCxLC) with optical detectors and MS. Isabelle has published over 10 papers in international peer viewed journals and is presenting at international conferences on a regular basis.
Paul Ferguson (AstraZeneca)
Paul is a separation science specialist at a multinational pharmaceutical company and leads the separation science strategy for their Development division working on both small and medium size (small peptides and oligonucleotide) therapies. He has worked in the pharmaceutical industry since 1999. Paul has particular interests in UHPLC, SFC, CE, chiral separations, formulated drug sample preparation, green analytical chemistry and method development over a range of separation techniques. He is a past winner of the Desty Memorial lecture prize (2002), a Fellow and Chartered Chemist in the RSC and is a visiting lecturer at Kings College London where he has lectured on the MSc Analytical Science for Industry course since its inception in 2009. He is also the current President of The Chromatographic Society in the UK having joined the Society committee in 2007.
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