GC, MDGC
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This article discusses biologically important steroid measurements which have, in the past, seen a lack of analytical sensitivity. Examples include determination of testosterone levels in women and children and estrogen levels in post-menopausal women. The article gives insight into the analysis of free testosterone at physiologically relevant levels in plasma ultra-filtrate, concentrating on the sensitivity, specificity, precision and accuracy advantages offered by GC-MS/MS.