Understanding HILIC separation and improving your results
Jul 28 2020 Read 1249 Times
Do you regularly work with HILIC columns?
Do you have a new project that requires you to try a HILIC separation?
You probably know that hydrophilic interaction chromatography (HILIC) is very different from reversed phase chromatography and the development of a robust and reproducible HILIC method can be a challenging process. Because HILIC is less well understood than other chromatographic methods the success of a method is often perceived to be predestined by the physicochemical properties of the analytes. This is something you can’t change, but can you use it to your benefit?
YMC have prepared a review highlighting influences on the separation mechanisms in HILIC mode as a helpful tool for method development and troubleshooting.
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