HPLC, UHPLC

  • Oligonucleotide Separations with RP Phases

Oligonucleotide Separations with RP Phases

Feb 04 2019 Read 204 Times

Therapeutic oligos represent an important area of research in the pharmaceutical industry today. These drug candidates are typically 8-50 nucleotides long and contain single-stranded DNA or RNA. Oligonucleotides are typically analysed using ion-pairing chromatography. Oligonucleotides are easily degraded via phosphodiester-cleaving enzymes. However, with a thiophosphate modified phosphate group, the stability significantly increases. Phosphorothioate modified oligos will have a stereocenter at each modified phosphate group, leading to 2n-1 diastereomers, all with an individual retention time in the chromatography. For a 20-nucleotide long oligo, that’s 524 288 species present. The partial resolution of these species results in peak broadening, further complicating chromatographic separation. To resolve the problem, ion-pairing agents with longer alkyl chains can be used.

In a study of the influence of stationary phase and ion-pairing agent on the separation of oligos, Kromasil Phenyl showed to be a very good choice when separating oligonucleotides in general, exhibiting very sharp peaks, and good selectivity.

View these applications on www.kromasil.com/oligos.

Read comments0

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.


Digital Edition

Chromatography Today - Buyers Guide 2019

December 2018

In This Edition Articles Fundamental Aspects - Improved Biomacromolecule Separations Using Superficially Porous Particles with a 1000 Å Pore Diameter - A Deep Characterisation of Monoclo...

View all digital editions

Events

China Lab 2019

Feb 26 2019 Guangzhou, China

Israchem

Mar 12 2019 Tel Aviv, Israel

ARABLAB 2019

Mar 12 2019 Dubai, UAE

MSB 2019

Mar 25 2019 Corvallie, OR, USA

Forum Labo Paris

Mar 26 2019 Paris, France

View all events