Trastuzumab High Resolution Separation with new HALO 1000Å C4 columns
Mar 22 2017 Comments 0
A new application note demonstrating the high resolution separation of the monoclonal antibody trastuzumab has been published by Advanced Materials Technology (AMT). The separation is acheived in approximately five minutes at 80°C, under gradient conditions with UV detection.
Trastuzumab (MW ~148 kDa) is used to treat breast cancer. Enhanced resolution of trastuzumab and its variants is acheived using a new HALO 1000Å C4 column. HALO 1000Å C4 columns contain the world’s first 2.7 micron superficially porous silica particles with 1000Å pores. The pore size and structure of these particles enable molecules to achieve complete penetration of the porous structure, allowing enhanced interaction with more of the bonded surface for better retention and selectivity. This results in improved separations of these types of large molecules.
This application note and a technical brochure are available from Hichrom Limited and demonstrate the ability of HALO 1000Å C4 to provide valuable detailed information not possible to acheive when using particles with smaller pore sizes. For more information or a copy of the technical brochure please email.
Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.
In this issue... Contents - Foreword by Trevor Hopkins Articles - The Emergence of Multiple Attribute Methodology (MAM) Workflows - Stationary Phase Selection for Achiral Separation ...
View all digital editions
Jan 24 2018 Cardiff, UK
Feb 05 2018 Dubai, UAE
Mar 18 2018 Dubai, UAE
Mar 18 2018 New Orleans, LA, USA
Mar 27 2018 Guangzhou, China