Columns (LC)

  • LC/MS Analysis of Pain Management Opioids with Ascentis® Express Biphenyl HPLC Columns

LC/MS Analysis of Pain Management Opioids with Ascentis® Express Biphenyl HPLC Columns

Nov 23 2016 Read 2120 Times

The improvement of pain management therapies is a persisting clinical need. Determination of benefits and side effects are critical studies to determine the efficacy and safety of existing and potential drug compounds. Conventional octadecyl stationary phases for HPLC analysis lack the selectivity to adequately resolve the components of complex pain management assays. The Ascentis Express Biphenyl is an alternative reversed-phase HPLC stationary phase chemistry with unique separation character providing retention and selectivity that are ideal for rapid, efficient drug and metabolite analysis using conditions that are compatible with MS detection.

Fused-Core® Technology
The Ascentis Express Biphenyl is the newest stationary phase available in the Ascentis Express Fused-Core line of U/HPLC columns. The extended aromatic system of the biphenyl moiety yields a more polar stationary phase, increasing retention for compounds that are challenging to analyse using standard C18 chemistry. The hydrophobic, aromatic, and steric properties are inherently orthogonal to alkyl bonded phase columns, providing unique selectivity. Narrow particle size distribution exhibited by the Fused-Core particles yields a more consistently packed bed while the thin, porous shell shortens the diffusion pathway of analytes, resulting in better mass transfer at high flow rates. The enhanced separation kinetics provides superior performance with high sample throughput.

LC/MS Analysis of Pain Management Opioids
The analysis of pain management opioids requires chromatographic resolution of key isobaric pairs that can’t be determined from MS detection alone. Ascentis Express Biphenyl successfully resolves all critical pairs in the opioid pain panel

Please click here for additional information and product availability.

Figure 1. LC/MS of Pain Management Opioids on Ascentis Express Biphenyl

Column Ascentis Express Biphenyl, 10 cm x 2.1 mm I.D., 2.7 µm (64065-U)
Mobile phase

[A] water with 0.1% formic acid

[B] acetonitrile with 0.1% formic acid

Gradient 10 to 20% B in 3 min; to 100% B in 3.5 min
Flow rate 0.3 mL/min
Column temp 30°C
Detector MS-TOF, ESI+, XIC
Injection 2 µL


Peak Compound m/z
1 Morphine 286
2 Oxymorphone 302
3 Hydromorphone 286
4 Naloxone 328
5 Codeine 300
6 Naltrexone 342
7 Oxycodone 316
8 Hydrocodone 300
9 cis-Tramadol HCl 264
10 Meperidine 248
11 Fentanyl 337
12 Bupprenorphine 468
13 (+/-)-Methadone 310


Digital Edition

Chromatography Today - March 2021

March 2021

In This Edition Modern & Practical Applications - Advancing Effective Glycan Analysis - Delivering the Power of Ion Mobility Spectrometry - Mass Spectrometry to the Point of Analysis - The...

View all digital editions



May 09 2021 Fort Worth, Tx, USA

e-PREP 2021

Jun 06 2021 Virtual event


Jun 21 2021 Dubai, UAE & Online


Jun 29 2021 Ljubljana, Slovenia

EuroAnalysis 2021 - cancelled

Aug 22 2021 Nijmegen, Netherlands

View all events