Columns (GC)

Are Mosquitos Getting Stronger? Chromatography Investigates

Oct 21 2016 Read 2090 Times

A recent study shows evidence for the hypothesis of cuticular hydrocarbon (CHC) production in Anopheles gambiae — one of the types of mosquito that cause malaria in Africa. The hypothesis suggested that increased CHC content could be one reason why mosquitoes are becoming more resistant to the various control mechanisms that we put in place.

Although a relatively minor pest in the West — Anopheles are a major cause of suffering in some areas of the world. Although malaria cases have reduced since 2000, in 2015 over 430,000 people died from malaria with an estimated 214 million cases — with Sub-Saharan Africa the focal point of many of these malaria cases and deaths.

Vector control — stop the mosquito

The reduction in malaria cases is partly due to control methods used — with the use of insecticides being the major contributor to the reduction. But mosquitoes are becoming increasingly resistant to the insecticides used, and there are not too many new insecticides to move to. This poses a big risk to the control of malaria carrying mosquitoes.

There are several reasons why mosquitoes become resistant to insecticides — much as the bacterial infections humans get become resistant to our current antibiotics. Misuse of insecticides includes poor spraying technique and poor use of the insecticide at the wrong concentration. The use of counterfeit insecticides is also an issue in preventing the spread of malaria. There is also evidence that mosquitoes are becoming resistant to several different types of insecticide — insecticide resistance is spreading.

Resistance, resistance, resistance

There are three types of mechanism at play in insecticide resistance:

  • Behavioural resistance — for example, after DDT was used to control indoor living mosquitoes, an outdoor type became dominant. Or the insects might simply move to the other side of a sprayed leaf.
  • Biochemical resistance — enzymes can detoxify the insecticide before it reaches its site of action, known as metabolic resistance. Alternatively, the target site of the insecticide can become modified so that the insecticide cannot bind to the protein or enzyme it is designed to target.
  • Physiological resistance — there are many examples of this, including the strengthening or thickening of the body wall to prevent penetration of the insecticide.

Cuticle thickening — blocking the insecticide

In a recent paper in PNAS — a team reports that insecticide resistant mosquitoes have an increased amount of cuticular hydrocarbons (CHC) when compared to non-resistance types. CHC are synthesized from fatty acids by insects and help to stop the insect from drying out. But, they have the additional benefit of helping to prevent the uptake of insecticide.

The team used gas chromatography to show that resistant mosquitoes had an extra 29% of CHC compared to non-resistant mosquitoes. They report finding almost 70 different multicomponent peaks that are related to a wide variety of organic compounds. The ability of GC to analyse multicomponent samples is discussed in the article, Better with Both: Leveraging Polar Selectivity and Polar Inertness using SLB®-IL (i-series) Capillary GC Columns.

Read comments0

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.


Digital Edition

Chromatography Today - March 2018 Volume 11 Issue 1

March 2018

In This Issue... Articles - Industrial Applications of Offline and Online Comprehensive Gas Chromatography - Improving Workflow in Group-Type GC×GC Analysis of Petrochemicals - Identifi ca...

View all digital editions

Events

ISCC & GCxGC 2018

May 13 2018 Riva del Garda, Italy

ASMS Conference

Jun 03 2018 San Diego, CA, USA

ACHEMA 2018

Jun 11 2018 Frankfurt, Germany

ISMM 2018

Jun 19 2018 Busan, Korea

PREP 2018

Jul 08 2018 Baltimore, MD, USA

View all events