Techniques for Biomolecule Characterisation, The Analysis of Polar Molecules, Superficially Porous (Core Shell) Phases

May 09 2017

These short seminars aim to provide busy chromatographers with the opportunity to keep up-to-date with the latest developments and key skills in the industry, without placing burdensome demands on hectic work schedules. If you are involved in practical, ‘hands-on’ chromatography and want to stay ahead of the competition, then these low cost, concentrated learning experiences are ideal for you. These seminars last approximately 1 to 2 hours and can be delivered as breakfast, lunch or evening seminars (or any other time!), either at your company or at a venue local to you, at a convenient date and time. Following the main seminar, an opportunity for delegates to stay on for an open Q&A session, or a more in-depth discussion about a specific problem or topic, can be arranged.

Techniques for Biomolecule Characterisation

This short seminar discusses chromatographic techniques suitable for the analysis of large molecular weight biomolecules. With an increased interest in the area of biotherapeutics and more regulations focused on these pharmaceuticals, possible methods to characterise biomolecules are required. Modes such as size exclusion (SEC), ion-exchange, hydrophobic interaction (HIC) and affinity chromatography will be covered for intact biomolecules. Techniques that may be more familiar to those analysts involved in small molecule analysis, such as reversed-phase LC-MS methods for protein digests will also be discussed, alongside new tools focused primarily on large molecules, for example SEC for monoclonal antibody monomer, fragment and aggregate separations. Course attendees will leave with a better understanding of the variety of techniques available to enable a more complete characterisation of biomolecules.

The Analysis of Polar Molecules

This course is suitable for analysts looking to explore different chromatographic techniques/modes applicable for the analysis of polar molecules. It will introduce you to a number of possible techniques that can be employed to retain polar molecules, generally hard to retain using C18 columns in reversed-phase solvent systems. It includes details of new column chemistries suitable for reversed-phase retention of polars, such as polar embedded columns, as well as alternative techniques including: ion pairing, mixed-mode, Hydrophilic Interaction Liquid Chromatography (HILIC), Aqueous Normal Phase (ANP) and Supercritical Fluid Chromatography (SFC). This course allows you to explore the various options available to expand the polarity range of molecules that can be retained and discusses how these complement standard reversed-phase methodology.

Superficially Porous Phases – Development and Benefits for HPLC/UHPLC

Superficially porous or core shell particle phases are becoming increasingly popular in today’s laboratory. This course will introduce you to the development of solid-core particle technology and the anatomy of a modern core shell particle. It will demonstrate how reduction in band broadening with these phases enables chromatographic separations to be performed with increased resolution and higher sensitivity, compared to fully porous phases. The range of particle sizes currently available for both small molecule and larger biomolecule separations will be discussed, along with parameters to be considered when transferring methods from fully porous to core shell phases. Examples will be shown to demonstrate how ultra-fast separations can be achieved without excessively high operating pressures. This course will enable you to develop faster HPLC/UHPLC analyses, thereby increasing laboratory productivity.


 



0118 930 3660
www.hichrom.co.uk
seminars@hichrom.co.uk

Digital Edition

Chromatography Today - September 2017 Volume 10 Issue 3

September 2017

In this issue: FUNDAMENTAL ASPECTS -  MS DETECTION - MS IONISATION TECHNIQUES - MS Atmospheric Pressure Ionisation Sources: Their Use and Applicability - Enhanced Peptide Identification Usi...

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