Chiral Method Development Screening Techniques: A practical guide and new approaches in LC-MS

Oct 14 2009 Read 3710 Times

Author: David S Bell, Denise Wallworth on behalf of Supelco

Free to read

This article has been unlocked and is ready to read.


Retention mechanisms in chiral separations are complex and tend to be unpredictable. As a consequence, generic screening techniques utilizing many chiral stationary phases (CSPs) are typically used in method development practices. Normal phase chiral HPLC is commonly used in Drug Discovery and whilst such procedures are well established, there is a strong trend towards more sensitive detection by MS that now calls for a review of mobile phase selection.

Additionally, the development of chiral pharmaceuticals has matured, generating larger and larger numbers of chiral molecules in late-stage product development. As a result, chiral LC-MS methods for DMPK and aqueous-compatible/LC-MS friendly methods for pharmaceutical (drug substance) analysis have become even more important.

The key to successful chiral method development is the availability of simple, rapid and reliable screening protocols that avoid the possibility of false positives/negatives. This article outlines a set of simple protocols that uses one of the well-known classes of chiral stationary phases (CSPs), the macrocyclic glycopeptides. CHIROBIOTIC phases are highly compatible with LC-MS and operate in a variety of different mobile phase types. LC-MS can be used further in method development itself. HPLC combined with tandem MS is often used for the simultaneous quantification of parent drug and metabolites, but it also has the potential for even faster chiral method development. In this article, we also investigate simultaneous chiral method development, injecting a composite set of chiral molecules using LC-MS-MS. By comparing the results from the probe set with that from a single solute, the effect on the screening protocol of simultaneous screening is investigated.

Free to read

This article has been unlocked and is ready to read.


Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment

Digital Edition

Chromatography Today - June 2020

June 2020

In This Edition Articles Fundamental Aspects - Separation of the 4 Enantiomers of the Fungicide Spiroxamine by LC-MS/MS - Optimising the Chiral Separation of the Pesticide Diniconazole...

View all digital editions


BMSS Annual Meeting - CANCELLED

Sep 08 2020 Sheffield, UK

Mass Spec 2020

Sep 14 2020 Virtual Symposium

ISC 2020 - POSTPONED TO 2022#

Sep 21 2020 Budapest, Hungary


Sep 27 2020 Saint-Malo, France

CE Pharm 2020 - ONLINE EVENT

Sep 28 2020 Bethesda, MD, USA

View all events