HPLC, UHPLC

Turning on the Heat in Liquid Chromatography - Tony Edge & Luisa Pereira

Sep 15 2010 Read 5062 Times

Author: Tony Edge & Luisa Pereira on behalf of Thermo Fisher Scientific (UK) Ltd

Free to read

This article has been unlocked and is ready to read.

Download

The use of temperature within liquid chromatography (LC) has traditionally been limited to isothermal studies up to 50° or 60°C. However, this does not realise the full potential that temperature can have within a liquid chromatographic system. In particular the ability to run with thermal gradients [1,2] or to run green LC [3,4], where there is no organic solvent used, is something that is not considered as routine within the liquid chromatography community.

The advent of ultra-high pressure liquid chromatography (UHPLC) has meant that chromatographers are becoming more aware of the extremes of chromatography, and the benefits that this can have in terms of the separations either due to efficiency gains or reduction in sample analysis times. The advent of the new phases has also led coincidentally to robustness in the column performance at elevated temperature allowing for more extreme chromatography to be tried. The advantages of this form of extreme chromatography are many-fold e.g. a reduction in viscosity leads naturally to a reduction in the operating pressure, allowing for much higher flow rates and thus reducing analysis times. It also leads to substantial changes in selectivity which can benefit the chromatographer. Interestingly the use of HypercarbTM, which is an ideally suited stationary phase for these extreme conditions, is seeing an increase in the number of applications [5,6].

Free to read

This article has been unlocked and is ready to read.

Download

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment





Digital Edition

Chromatography Today - June 2020

June 2020

In This Edition Articles Fundamental Aspects - Separation of the 4 Enantiomers of the Fungicide Spiroxamine by LC-MS/MS - Optimising the Chiral Separation of the Pesticide Diniconazole...

View all digital editions

Events

BMSS Annual Meeting - CANCELLED

Sep 08 2020 Sheffield, UK

Mass Spec 2020

Sep 14 2020 Virtual Symposium

ISC 2020 - POSTPONED TO 2022#

Sep 21 2020 Budapest, Hungary

MSB 2020 - NEW DATES

Sep 27 2020 Saint-Malo, France

CE Pharm 2020 - ONLINE EVENT

Sep 28 2020 Bethesda, MD, USA

View all events