HPLC, UHPLC

High temperature liquid chromatography - a brief review about an emerging technique - Thorsten Teutenberg

Sep 09 2010

Author: Thorsten Teutenberg on behalf of Unassigned Independent Article

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This review is focused to present a general overview about high-temperature liquid chromatography. It starts with a brief definition and then explains the necessary requirements to make use of this emerging technique. Also, the advantages of high-temperature liquid chromatography such as the reduction in the mobile phase’s viscosity and the possibility to replace toxic organic solvents with water are outlined.

Furthermore, the influence of temperature on selectivity is demonstrated. This means that temperature gradients can be integrated into method development to optimize the resolution of critical peak pairs.

In the last few years, there is renewed interest to explore the full potential of temperature in liquid chromatographic separations. Why is this? Although it might sound curious, temperature can be regarded as a universal parameter in liquid chromatography. Temperature influences almost every other parameter which can be used to optimize a separation in terms of speed and resolution [1,2]. However, this is only one aspect. There are some special hyphenation techniques which rely on the use of a water-only mobile phase [3-6]. In this case, temperature is the only option to change the solvent properties of water, which becomes more like an organic solvent with increasing temperature [7]. Before going into further detail, it is useful to define the practical temperature range in hightemperature liquid chromatography...
 

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