HPLC, UHPLC

Holistic LC strategies, from UHPLC to HPLC and back

Jun 18 2010

Author: Adrian Clarke, John Nightingale, Partha Mukherjee and Patrik Petersson on behalf of Unassigned Independent Article

Free to read

This article has been unlocked and is ready to read.

Download

UHPLC has recently become a powerful tool in pharmaceutical analysis, specifically for method development, routine and rapid analysis. However, there are often limited numbers of UHPLC instruments available within internal and external customers and they do not always have access to this technology. This article gives an overview of the development and application of a holistic LC strategy to facilitate the implementation of UHPLC within a global Pharmaceutical Development function. An approach is being adopted that will enable the translation (geometric scaling) of chromatographic methods from “core” generic UHPLC methods with <2 μm columns to HPLC with ≥3 μm columns. There are requirements for UHPLC to HPLC transfer and practical issues to consider, specifically: instrument dwell volume differences, ensuring consistent selectivity across different particle sizes, frictional heating effects and pressure-induced retention effects. Finally the article demonstrates examples of UHPLC to HPLC translations and the application in AstraZeneca R&D projects.

 

Free to read

This article has been unlocked and is ready to read.

Download


Digital Edition

Chromatography Today - September 2021

September 2021

In This Edition Modern & Practical Applications - Analysis of Cannabidiol in CBD products by SFC-CD-MS - Faster results with SFC for the analysis of choline and acetylcholine in rat cerebros...

View all digital editions

Events

ASMS Conference - NEW DATES

Oct 31 2021 Philadelphia, PA, USA

ISPPP 2021

Nov 07 2021 Porto, Portugal

JASIS 2021

Nov 08 2021 Chiba, Japan

HUPO Reconnect 2021

Nov 15 2021 Virtual event

Arabchem - NEW DATES

Nov 15 2021 Dubai, UAE

View all events