Gel permeation chromatography (GPC), also known as size exclusion chromatography (SEC) is a technique for obtaining information on the molecular weight distributions of polymers. After polymer type (c...
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The area of biomolecular characterisation for molecular weight determination has increased rapidly with the requirements for a complete picture of the behaviour of biomolecules in solution being both...
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About 5 years ago, [1] the particle size of packing media in HPLC columns was reduced to 2 µm or smaller. This new column technology named U-HPLC or UPLC offers much better resolution and column effic...
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Stimulated by the sustainability agenda, the polymer industry is actively engaged in developing new materials that combine excellent performance with low carbon footprint. Molecular weight is a defini...
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Introduction
The requirement for high throughput analytical tools that can readily separate, purify and analyse ribonucleic acids (RNA) are assuming increasing significance with the recent discoverie...
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Abstract
RNAi products require both single strand and duplex impurity assays. Duplex assays should allow titration of guide or passenger strands that may be in excess. These assays are chromatographi...
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Polymers used in engineering applications are particularly difficult to analyse - they are generally crystalline, tough, and often require elevated temperatures or exotic solvents for dissolution and...
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Delivery of pure, dry, free flowing solids by a customer focused, medicinal chemistry purification service, using a Lean Sigma approach.
Over the past few years the concept and benefit of Lean Sigm...
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Over the past few years, preparative chromatographic separation of racemic mixtures into their individual enantiomers has become an integral part of the development process for new drug entities. This...
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Liquid-Liquid Chromatography instrumentation (L-LC), also referred to as Counter Current Chromatography (CCC), and Centrifugal Partition Chromatography (CPC), design has been instigated and, champione...
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As the bio-pharmaceutical industry matures, terms like “cost of goods” are becoming more and more important. Up to now, strain optimisation for high productivity and upstream purification were the bot...
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Retention mechanisms in chiral separations are complex and tend to be unpredictable. As a consequence, generic screening techniques utilizing many chiral stationary phases (CSPs) are typically used in...
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Chromatographic analysis and purification of optically active compounds are still areas with large potential for improvement. In the analytical field, chiral stationary phases (CSP) with better perfor...
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This article discusses factors important to operating a fast (high throughput) and efficient HPLC/SFC method development and prep purification laboratory. Discussion will include procedures for quickl...
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Developing simple and straightforward reversed phase chiral HPLC separations coupled with highly sensitive MS detection is highly needed for conducting drug metabolism and pharmacokinetic studies of s...
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