The introduction by several chromatography column manufacturers of sub-2 µm fully-porous as well as core-shell sub-3 µm UHPLC columns over the last several years have profoundly impacted the chromatog...
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A seminal paper was written by Manz et al.
(1) identifying the use of possibilities of using ‘chip based technology’ within an analytical instrument. When did you as a scientist first think that ‘chi...
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Monoclonal antibodies (mAbs) are an important class in the group of new biological entities with about 30 antibody drugs licensed for treatment of various diseases [1]. These glycoproteins bear comple...
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There is growing environmental concern regarding the health impact of trace levels of pharmaceuticals and personal care products (PPCPs) in water resources. Although the term PPCP was only recently co...
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The use of countercurrent chromatography (CCC) as a preparative technique is seen to occupy a niche area of separation science and is largely used to isolate natural products. The technique has howeve...
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Introduction
Water Condensation Nucleation (or Water Condensation Particle Counting) is a technology originally developed to detect aerosol particles in the application field of environmental monitor...
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A method was developed to analyse three model aminoglycosides (neomycin, apramycin, and kanamycin) using a zwitterionic HILIC (hydrophilic interaction liquid chromatography) stationary phase and mass...
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Today Hydrophilic Interaction Liquid Chromatography (HILIC) is by far the fastest growing HPLC separation mode, currently being used by almost 20% of the HPLC instrument owners [1], although the relat...
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A method for the analysis of dextran-1 has been developed using hydrophilic interaction liquid chromatography (HILIC) with charged corona aerosol detection (CCAD) as a potential alternative to the siz...
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Many liquid chromatography methods are developed using organic mobile phase additives which allow compatibility with mass-spectrometric (MS) detection. However, these types of additives often give hig...
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Synthetic Oligonucleotides as Therapeutic Medicines
Synthetic oligonucleotides are an exciting new class of biomolecules capable of treating many disorders, which are currently not amenable to exis...
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In developing new analytical instruments and in particular chromatography instrumentation there is a need to modify or manufacture components to perform experiments and initiate the innovation. Usuall...
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The present paper describes a multi-factorial optimization of three critical HPLC method parameters, i.e. gradient time (tG), temperature (T), and ternary composition (B1:B2) based on twelve experimen...
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TYPE C™ silica is a relatively new chromatographic material that has been finding ever-increasing use in the last few years. The properties exhibited by these stationary phases are often significantly...
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This article aims at presenting the fundamental steps when implementing a two-dimensional on-line separation (2D-LC) of complex samples.
Firstly, an approach is proposed to make the search for orth...
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