A new method for the simultaneous quantification of multiple phytohormones and metabolites has been found by researchers at the Huazhong Agricultural University in Wuhan, China.
The UFLC-ESI-MS method has been identified as being a convenient, sensitive, reliable, and inexpensive method for the quantification of multiple phytohormones and metabolites in comparison to existing methods. Results from the study results will provide a basis for understanding the molecular mechanisms of rice defence responses.
Ultra fast liquid chromatography-electrospray ionization tandem mass spectrometry (UFLC-ESI-MS) was used to provide a convenient procedure for preparing filtrated crude extracted samples and a sensitive quantification assay.
This allows for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid, hormone conjugates, IAA-aspartic acid, JA-isoleucine, and methyl jasmonate, and phytoalexins, momilactone A, naringenin, and sakuranetin is achievable through this method.
The researchers claim this research has led to a significant understanding of the dynamic profiles of defense-related phytohormones, hormone metabolites, and phytoalexins in the interaction of rice with Xanthomonas oryzae pv. oryzae (Xoo).
Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, which is one of the most devastating diseases of rice worldwide.
Posted by Ben Evans